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© Kamla-Raj 2005 Int J Hum Genet, 5(1): 49-55 (2005)
Amplification of c-myc Locus is Independently Associated with the Deletions of Chromosome 8p in Breast Carcinoma
Nilanjana Bhattacharyaa, Neelanjana Chundera, Anup Royb, Niyaz Alama, Susanta Roychoudhuryc and Chinmay Kumar Pandaa *
aChittaranjan
National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700 026, bDepartment of Pathology, Medical College and Hospital, Kolkata 700 073, West Bengal, India cHuman Genetics and Genomics Division, Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700 032, West Bengal, India
Keywords Breast carcinoma; chromosome 8; c-myc locus; pal-1; mlvi-4
ABSTRACT Attempts have been made in this study is to find out the mechanism of c-myc gene activation in breast carcinoma (CaBr) by analyzing alterations (rearrangement/amplification) in the ~580 Kb surroundings of this gene. The alteration in the c-myc locus was correlated with the deletions in chromosome (chr.) 8p to find if there is any association between the two phenomenons. The c-myc locus alteration was analyzed by Southern hybridization using the pal-1/ c-myc/ mlvi-4 probes. Overall, amplification in the c-myc locus was seen in 26% of the samples with 22% in the pal-1 region, 19% in the c-myc gene and 7% in the mlvi-4 region. This indicates that the c-myc gene activation may occur due to the amplification in the pal-1 region located 550 Kb 5’ and mlvi-4 region located 20 Kb 3’ of c-myc. About 42% of the samples showed loss of heterozygosity (LOH) in ³ 40% of the microsatellite markers tested. Atleast 21% of the samples showed co-alterations in both arms of chr.8. No significant association was observed between the amplification in the c-myc locus and deletions in chr.8p. Thus the deletions in chr.8p and the amplification in the c-myc locus are independently associated with the development of CaBr.
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