© Kamla-Raj 2004                                                                        Int J Hum Genet, 4(4): 249-255 (2004)

Studies on the Genotoxic Effects of Anticancer Drug

Carboplatin in in vivo Mouse

K.K. Vijayalaxmi and Marie Prem D’Souza

Department of Studies in Applied Zoology, Mangalore University

Mangalagangotri  574 199, D.K., India

KEYWORDS Genotoxicity; antitumor drug; fetal liver; transplacental; micronucleus

test; sperm abnormality

ABSTRACT Genotoxicity of platinum antitumor agent carboplatin (Cbp) was investigated by using mouse in vivo test system. Three different doses of carboplatin viz., 30, 60 and 90 mg/kg b.w were used for the experiment. Fetal liver micronucleus (MN) test and sperm abnormality assay were  used as the test parameters. Cyclophosphamide (50 and 100mg/kg b.w) was used as positive control and distilled water formed the negative control. Both the chemicals were administered intraperitoneally to experimental animals.  MN and sperm preparations were made at 24 hr. and 35 days respectively. Carboplatin induced significant MN both in the fetal liver and the maternal bone marrow at different doses. It also induced high frequency of abnormal sperms at higher doses and the effect was significant at all the 3 doses tested.

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