|
|
|
© Kamla-Raj 2004 Int J Hum Genet, 4(1): 65-69 (2004)
Cytogenetic Analysis of Radiotherapeutic and
Diagnostic
P. K. Gadhia, Nehal Shah, Sweta Nahata, Swati Patel, Krupa
Patel,
Department of Biosciences, South Gujarat University, Surat 395 007, Gujarat, India
Keywords Radiation workers; human chromosomes; CAs; SCE; micronuclei
Abstract The study group comprised of 12 occupationally exposed “radiotherapeutic and diagnostic workers”, working since last 12 years on an average (service duration 3 to 20 years), with 12 age and sex-matched controls not exposed to any kind of radiation and belonging to same socio-economic status as the radiation workers. Cytogenetic end points studied were CAs (Chromosomal aberrations), SCE (Sister chromatid exchange) and MN (Micronuclei). Hematological parameters were also studied. In addition, co-mutagenic/synergistic in vitro effects of known mutagen Mitomycin-C (MMC) on lymphocytes of these workers were evaluated. Results revealed a significant increase in dicentric (P < 0.05) as well as MN (P< 0.01) among radiation exposed workers when compared to controls. By contrast, no change in SCE frequencies and hematological parameters were observed. After in vitro MMC treatment CA (mainly dicentric and ring) increased significantly in lymphocytes of radiation exposed workers. Based on these observations, a preliminary indication of the study could be that long term low level radiation exposure may probably damage the genetic constitution of an individual.
|
|
|
