© Kamla-Raj 2002                                                                           Int J Hum Genet, 2(4): 273-274 (2002)

Use of Porcine Serum in Lymphocyte Culture

R. victor¹,  Pallavi a. rao¹,  Pushpa Vasudev¹,  D. Sebastian¹, 
Veena A. Rao¹  and  Sayee Rajangam² 

1. Genetic laboratory s-dac lal bagh nursing home, jayanagar,

bangalore 560 034, Karnataka, India

2. Division of human genetics, department of anatomy, st.john's medical college

 bangalore 560 034, Karnataka, India

Key Words  lymphocyte culture; porcine serum; GTG banding; karyotyping

Abstract In lymphocyte culture work, apart from human serum, animal serum was in use for a long time. Foetal calf / bovine ,goat serum were the common ones used. Porcine serum has not been commonly used though it is said that the constituents of the porcine  and human blood are more of the same. Porcine serum in lymphocyte culture for chromosomal studies has been tried and found to be good. It worked better than the goat serum. GTG bands were well seen in the metaphase spreads. Hence, it is suggested that porcine serum could also be used in setting up peripheral lymphocyte culture for karyotyping.

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