© Kamla-Raj 2002                                                                                Int J Hum Genet, 2(1): 1-4  (2002)

Microdissection and Reverse Painting. Reevaluation of Three

Cases with Structural Abnormalities in Chromosome 13

Purnima Kumarathas¹,  Peter K.A. Jensen²,  Mette Houman¹  and  Ursula Friedrich¹

1. Department of Human Genetics, University of Aarhus, Denmark

2. Department of Clinical Genetics, Aarhus University Hospital, Denmark

Key words microdissection; deletion of chromo-some 13; phenotype-genotype correlation.

Abstract  Three patients with different deletions of chromosome 13 were reexamined. The abnormal chromosomes were microdissected. The DNA from these chromosomes was DOP amplified, labelled with Biotin and used for reverse banding by FISH. The precise break-points in chromosome 13 were defined which is a prerequisite for delineation of the different deletion phenotypes.

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