Kamla-Raj 2003                                                                             Int J Hum Genet, 3(1): 45-50 (2003)

 

 

Study of Genetic Polymorphism at D21S11 and D21S215 loci in the

Jat Sikh Population of Punjab

 

I.S. Sidhu1, K. Kaur1, V.K. Sarhadi2, D.S. Joshi3, R. Mukhopadhaya3,
S.K. Mahajan3 and A.J.S.
Bhanwer1

 

1. Department of Human Genetics, Guru Nanak Dev University, Amritsar 143 005,

Punjab India

2. Centre for Genetic Disorders, Guru Nanak Dev University, Amritsar 143 005,

Punjab India

3. Molecular Biology and Agriculture Division, BARC, Trombay, Mumbai 400 085,

Maharashtra, India

 

Key Words Microsatellite; PCR; genetic polymorphism; DNA; primers

 

Abstract  Microsatellites, 1-6 bp long tandem repeats, are new class of polymorphic DNA markers and has many advantages over other DNA markers like minisatellites, VNTRs etc. due to which, these become markers of choice for studying genetic polymorphism at population level, forensic investigations, linkage studies etc. Genotyping of populations can provide genetic basis for affinities among different ethnic groups. Polymorphism revealed by microsatellites could be used to study the micro-evolutionary trends among populations. In the present study polymorphism at two microsatellites markers D21S11 (four bp repeat) & D21S215 (two bp repeat) studied among the Jat Sikh population of Punjab, India. Eight alleles of D21S11 and four alleles of D21S215 with heterozygosities 82.2% and 66.6% were observed respectively. Allelic polymorphism was compared with the data available for other Indian populations and Caucasoid population at D21S11 locus.

 


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