© Kamla-Raj 2001                                                                        Int J Hum Genet, 1 (3): 179-182 (2001)

Distribution of Apolipoprotein E (APOE) Genotypes
in a Siberian Female Population Sample

A. Belkovets¹, S. Kurilovich M¹, Dolgich¹ and D.P. Agarwal²

1. Institute of Internal Medicine, Novosibirsk, Russia

2. Institute of Human Genetics, University of Hamburg, 22529 Hamburg, Germany

Key Words Apolipoprotein E; polymorphism; allele frequency; Siberia.

Abstract   Variations at the apolipoprotein E  (APOE) locus influence lipid and lipoprotein levels in the normal population, and are  associated with premature coronary artery disease. In the present study, the APOE genotypes and allele frequencies in 299 women from Novosibirsk (West Siberia) were determined. APOE  genotypes were detected by restriction isotyping after amplification of the genomic DNA by PCR. APOE*3 was found to be the predominant allele (allele frequency = 0.769). The frequency of APOE*2 and APOE*4 alleles were found to be 0.048 and 0.182, respectively. The results are compared with those reported for major human populations of the world. Moreover, genotypic relationship with quantitative lipid levels (total cholesterol, high-density lipoprotein cholesterol, and triglycerides) were examined. Subjects with APOE*2 allele showed much lower total choletsterol and HDL values as compared to APOE*4 allele carriers supporting the notion that E*4 allele is a susceptibility factor for cardiovascular diseases.

Home                         Back